中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (7): 1057-1062.doi: 10.3969/j.issn.2095-4344.2015.07.014

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

艾烟溶液干预人肺腺癌细胞活性氧和超氧化物歧化酶的活性

窦传字1，吴焕淦1，王硕硕2，马晓芃1，黄 艳1，赵继梦3，胡智海2，刘慧荣1，崔云华1，周次利1，赵 琛4   

  1. 1上海中医药大学上海市针灸经络研究所，上海市 200030； 2上海中医药大学附属上海市中西医结合医院，上海市 200082； 3上海中医药大学，上海市 201203；4上海中医药大学针灸推拿学院，上海市 201203
  • 出版日期:2015-02-12 发布日期:2015-02-12
  • 通讯作者: 赵琛，博士，主任医师，上海中医药大学针灸推拿学院，上海市201203
  • 作者简介:窦传字，男，1984年生，山东省青岛市人，汉族，2013年上海中医药大学毕业，博士，助理研究员，主要从事针灸临床与基础研究。
  • 基金资助:

    上海市卫生局科研课题(20134239)；国家自然科学基金青年基金项目(81102637)；国家重点基础研究发展计划(973计划)(2009CB522900)

Activities of reactive oxygen species and superoxide dismutase in human lung adenocarcinoma cells impacted by moxa smoke solution

Dou Chuan-zi1, Wu Huan-gan1, Wang Shuo-shuo2, Ma Xiao-peng1, Huang Yan1, Zhao Ji-meng3, Hu Zhi-hai2, Liu Hui-rong1, Cui Yun-hua1, Zhou Ci-li1, Zhao Chen4   

  1. 2012031Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China; 2Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China; 3Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 4Acupuncture and Tuina College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2015-02-12 Published:2015-02-12
  • Contact: Zhao Chen, M.D., Chief physician, Acupuncture and Tuina College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • About author:Dou Chuan-zi, M.D., Assistant researcher, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
  • Supported by:

    the Science and Technology Research Project of Shanghai Health Department, No. 20134239; the National Natural Science Foundation of China for the Youth, No. 81102637; the National Basic Research Program of China (973 Program), No. 2009CB522900

PDF

595

被引次数

10

摘要：

背景：艾灸生成物的安全性研究是近年来灸法领域的关注热点，探讨艾烟成分对人体器官、组织、细胞的生物学效应是研究艾灸安全性的重要方向。研究表明艾烟溶液可引起肺泡Ⅱ型上皮细胞A549凋亡，但具体机制尚不清楚。

 

目的：观察艾烟采集物对肺泡Ⅱ型上皮细胞A549生长抑制率、细胞内活性氧、超氧化物歧化酶的影响，探讨艾烟采集物对A549的氧化损伤机制。

 

方法：采用便携式空气采样器采集艾条燃烧产生的烟气颗粒物，加入DMEM培养基分别配制质量浓度0.5，0.4，0.3，0.2，0.1，0.05，0.025，0.01 g/L的艾烟溶液干预A549细胞。采用细胞计数试剂盒(CCK-8)检测细胞生长抑制率，2'，7'-二氯荧光黄双乙酸盐法观察细胞内活性氧信号强度，分光光度法测量并计算细胞超氧化物歧化酶活性。

 

结果与结论：不同质量浓度艾烟溶液干预A549细胞4，6，8 h结果均显示，0.4，0.5 g/L艾烟溶液对A549细胞的生长抑制率高于0.01，0.025 g/L组(P < 0.05)。质量浓度0.05，0.1 g/L艾烟溶液可降低肺A549细胞内活性氧的含量，但差异无显著性意义(P > 0.05)；高质量浓度0.4 g/L艾烟溶液可增加肺A549细胞内活性氧水平(P < 0.01)，降低超氧化物歧化酶活性(P < 0.01)。结果证实，艾烟对A549细胞具有生物学活性，对细胞的过氧化作用可能是高浓度艾烟溶液抑制A549细胞生长的重要机制。

 


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 组织工程, 艾烟, 肺泡Ⅱ型上皮细胞A549, 氧化损伤, 安全性, 细胞培养, 生长抑制, 活性氧, 超氧化物歧化酶, 国家自然科学基金

Abstract:

BACKGROUND: The safety study of moxa smoke has been the focus of attention in the moxibustion research in recent years. As an important direction, the effects of moxa smoke on the human organ, tissue and cells have been discussed. Studies have shown that the solution of moxa smoke can induce apoptosis of human lung adenocarcinoma cells (A549), but the specific mechanism is still unclear.

 
OBJECTIVE: To observe the influences of moxa smoke on growth inhibition, intracellular reactive oxygen species and superoxide dismutase of A549 cells, and to explore the oxidative damage mechanism of moxa smoke on A549 cells.
METHODS: Moxa smoke was collected by a portable air sampler, and dissolved in DMEM to different concentrations, as 0.5, 0.4, 0.3, 0.2, 0.1, 0.05, 0.025, 0.01 g/L, which was used to culture A549 cells. Cell Counting Kit-8 method was employed to test cell growth inhibition rate, 2’,7’-Dichlorofluorescin diacetate method was employed to determinate reactive oxygen species intensity, and spectrophotometric method was employed to measure and calculate enzymatic activity of superoxide dismutase.
RESULTS AND CONCLUSION: The results of different concentrations of smoke solution intervening A549 cells in 4, 6 and 8 hours showed that the concentration of 0.4 g/L and 0.5 g/L on A549 cells growth inhibition rate was higher than 0.01 g/L and 0.025 g/L (P < 0.05). Low concentration of moxa smoke (0.05 g/L and 0.1 g/L) could decrease the intracellular levels of reactive oxygen species, but there was no statistically significant difference between them (P > 0.05); high concentration of moxa smoke (0.4 g/L) could increase intracellular levels of reactive oxygen species (P < 0.01), and decrease the activity of superoxide dismutase (P < 0.01). Moxa smoke is shown to have biological activity on A549 cells, and the mechanism of high-concentration moxa smoke effect on cell growth inhibition may be attributed to peroxidation.


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: Tissue Engineering, Safety, Cells

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